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KMID : 0545120000100010091
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 1 p.91 ~ p.94
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Expression of Modified Green Fluorescent Protein in Suspension Culture of Taxus cuspidata
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KIM, CHANG HEON
KIM, KYUNG IL/CHUNG, IN SIK
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Abstract
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The suspension cells of Taxus cuspidata were transformed with Agrobacterium tumefaciens harboring binary vector pCAMBIA1302 encoding mgfp. Transient transfection efficiency was compared by using the fluoremetric measurement. The transient transfection efficiency was improved by transformation with DMSO and/or sonication treatment. Optimum conditions for DMSO and sonication treatment were 3% and 30sec, respectively. Selection and maintenance of transformed cells were continued for 3 months. An insertion of the mgfp gene in transformed cells was detected by PCR and an expression of GFP confirmed by the western blot analysis.
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